Fig. 8

ISH in killifish tail fin yields better signal to noise ratio when fixed with PFA + formic acid. A Morphology of the tail fin in the African killifish N. furzeri. Amputation site is indicated by red dashed line. B Chromogenic in situ hybridization of the injury-responsive gene fstl1. 1 dpa and 3 dpa tail fin tissues fixed with 4% PFA and probed for fstl1.C ISH for fstl1 in tail fins fixed with 4% PFA + formic acid. Brightfield images were taken with a stereomicroscope. Scale bars are 500 μm