Fig. 3

HRJD expression compromises regeneration in the adult midgut. A Schematics of the Drosophila adult midgut. The adult midgut is a pseudostratified epithelium, in which intestinal stem cells (ISCs) generate both absorptive enterocytes (ECs) and secretory enteroendocrine cells (EEs) through progenitor cell enteroblasts (EBs) and EE progenitor cells (EEPs). B Experimental scheme for ISC/EB specific induction of HRJDs under the esg-Gal4 driver in the adult stage. Adult flies were transferred to 29 °C at day 3 to induce HRJD expression. C–E Survival curve during paraquat (C), DSS (D), and sucrose (E) feeding. F Representative images for PH3 staining upon paraquat feeding. G Quantification of PH3 positive cells in posterior midguts. N.S., not significant: P > 0.05, *P < 0.05, **P ≤ 0.01. Log-rank test. n indicates the number of flies (C–E) and midguts (G). Scale bars: 50 μm. See also Additional file 1: Fig. S2