Fig. 1

HRJD expression in whole body does not affect gross morphology. A Phylogenetic tree of HRJD conservation. Green indicates species that possess HRJD gene(s) and gray indicates species that have lost HRJD gene(s). High regenerative ability indicates that the species can regenerate their whole body or anterior/posterior body parts, and low regenerative ability indicates that the species can only regenerate their appendage (limbs, tails, fins) or much smaller scale of tissues/organs. We referred to Cao et al. (2019) for the definition of regenerative ability [13]. B Protein sequence of HRJDs used in this study, which derived from Dugesia japonica (DjHRJDa/b, hereafter simply described as HRJDa/b). Conserved residues characteristic of the JmjC domain are shown. C Schematics of genetic experiments for whole body induction of HRJDs. The Gal4-UAS system enables gene expression downstream of the UAS sequence, which is regulated by the transcription factor Gal4 [15]. In this case, the ubiquitously active Act5C promoter is used for Gal4 expression. D Representative images of mature adults for whole body expression of HRJDs. E Whole body induction of HRJDs did not change body weight of mature adults. F Representative images of adult wings. G Whole body induction of HRJDs did not change wing size. H Representative images for adult heads. I Whole body induction of HRJDs did not change eye size of adult flies. N.S., not significant: P > 0.05. One-way ANOVAs with post hoc Tukey test. n indicates the number of flies examined. See also Additional file 1: Fig. S1